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November 2022: Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory in Wheat

Dear PhotosynQ User,

Welcome to the PhotosynQ community newsletter!

This month we have the International Journal of Molecular Sciences Research Article on "Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.)". This study explains the importance of studying drought tolerance in wheat and drought memory responses of wheat plants that were earlier exposed to drought conditions. We hope you enjoy reading our selected news and please stay safe and healthy! Best, PhotosynQ Team

Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.)

Hong Yue, Haobin Zhang, Ning Su, Xumin Sun, Qi Zhao, Song Weining, Xiaojun Nie, and Wenjie Yue.

Wheat is the most widely cultivated worldwide crop and contributes to 30% of world food intake. Wheat yields must be increased by 60% in 2050 to meet demand though drought imposes a significant challenge in accomplishing this target. Plants can remember abiotic stresses from their earlier encounters through structural, genetic, and biochemical modifications. This phenomenon is generally known as stress memory which can provide a substantial amount of tolerance to subsequent exposure to the stress in later stage of the life cycle. The authors of this study identified 195 candidates of drought memory-related miRNAs, 34 of which were validated.

The researchers used MultispeQ to measure Phi2 of the experimental wheat plants to assess the photosynthetic difference. The experiments included the wheat plants of control group (CG), direct drought (DD) exposed, and drought memory (DM). The measurements were taken 0, 24, 48 and 72 hrs after drought-like conditions.

Figure 1. (A) Drought resistance detection of seedlings in three groups; (B) Trileaf stage seedlings were divided into three groups, control (CG), direct drought (DD), and drought memory (DM) and provided with the respective treatments to mimic drought memory (DM only) and drought (all 3 groups); (C) Proline content comparison amongst the three groups after treatment ; (D) Comparison of the efficiency of photosystem II (Phi2) amongst the three groups after treatment for 0, 24, 48, and 72 h. Green marks the efficiency of CG, blue of DM and red of DD.

As the data distributions of Figure 1D demonstrate, the photosynthetic efficiency of the DM group is significantly higher than the Direct Drought group and very close to that of the Control Group plants.

This project highlights the value of MultispeQ as a tool for non-destructive measurement of photosynthesis in wheat plants response testing for drought and how drought tolerancemay be better bred in future varieties.



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